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KMID : 0378019710140110109
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New Medical Journal
1971 Volume.14 No. 11 p.109 ~ p.117
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An Autoradiographic Study of Effects of Puromycin on Protein Synthesis of Submandibular Gland of Mice using Leucine-C¹⁴
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Abstract
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Male Balb C/Strain were used in all experiment. They ranged in weight from 18 to 23 gm. Puromycin dihydrochloride was injected intraperitoneally in aqueous solution, in a concentration of 2mg. /ml. , which had been neutralized with naoh.
Two doses of 80mg/kg body weight each were given on day 1 intervals.
Following the last injection, 3 pairs each were sacrificed on days 4, 6, 10 and 15. Thirty minutes prior
to sacrifice 49c/gm body weight of leucine-C" (Specific Activity 278mc/mM) were injected.
For the light microscopic studies, tissue were fixed 2% paraformaldehyde and sections were stained
with toluidine blue o.
The purpose of the present study was to determine, by means of quantitative autoradiography, the effect of puromycin on the protein synthesis of submandibular gland cells.
1. Following administration of puromycin to mice for 10 days, many of the acinar cells are lack the normal basophilia, increase of the vacuoles and the nuclear pyknosis.
2. We have observed that the protein synthesis as expressed by the incorporation of leucine-C" into submandibular gland cells is suppressed during first 4 days after the puromycin injections, but was significantly suppressed by 10 days. The grain number in puromycin-treated animals increased to a level similar to that of the controls by 15 days.
3. It is concluded that the synthesis of overall cytoplasmic proteins is temporarily impaired after puro-￢mycin injections in submandibular gland cells of mice.
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